ANALYSIS OF WATER SALINITY LEVEL OF FREQUENCY MOLTING IN VANNAMEI SHRIMP (LITOPENAEUS VANNAMEI) ABLATED

Authors

DOI:

https://doi.org/10.33603/jgst.v4i2.3436

Abstract

Media salinity is a masking factor that plays an important role in controlling the process of shrimp molting and osmoregulation. Both of them are ecophysiological factors for shrimp life, so it is necessary to know the optimum level or range of media salinity for each phase of the molting phase changes in the stage and osmoregulation phase. This study was conducted to examine the molting frequency of adult L. vannamei which was ablated and cultivated at various levels of salinity. This research was carried out for 60 days. This study uses experimental laboratory methods with a systematic randomized design (RAS) with 4 treatments and 3 replications in each treatment. The treatments tested were salinity with S1 treatments (10 ppt, 289.20 mOsm / l postmolt H2O isosmotic), S2 (15 ppt, 432.80 mOsm / l H2O initial intermolt isosmotic), S3 (25 ± 1 ppt, 725, 15 mOsm / l H2O isosmotic final intermolt) and S4 (29 ± 1 ppt, 820.10 mOsm / l H2O isosmotic molt). Data were analyzed with ANOVA. Meanwhile, the difference in effect between treatments was obtained through Duncan's multiple area test. ANOVA results showed that various levels of isoosmotic media salinity at various molting phases had an influence (p <0.05) on molting frequency. The best value of molting frequency was in the S4 treatment (29 ± 1 ppt) (close to isoosmotic molt) 10 times. In the maintenance of L. vannamei which is affixed, it should pay attention to the needs of isoosmotic media, namely media with a salinity of 25 ± 1-29 ± 1 ppt (final intermolt isoosmotic range / premolt to molt). the difference in effect between treatments was obtained through Duncan's multiple region test. ANOVA results showed that various levels of isoosmotic media salinity at various molting phases had an influence (p <0.05) on molting frequency. The best value of molting frequency is in the treatment of S4 (29 ± 1 ppt) (close to isoosmotic molt) 10 times. In the maintenance of L. vannamei which is affixed, it is better to pay attention to the needs of isoosmotic media, ie media with a salinity of 25 ± 1-29 ± 1 ppt (final intermolt isoosmotic range / premolt to molt). the difference in effect between treatments was obtained through Duncan's multiple region test. ANOVA results showed that various levels of isoosmotic media salinity at various molting phases had an influence (p <0.05) on molting frequency. The best value of molting frequency is in the treatment of S4 (29 ± 1 ppt) (close to isoosmotic molt) 10 times. In the maintenance of L. vannamei which is affixed, it is better to pay attention to the needs of isoosmotic media, ie media with a salinity of 25 ± 1-29 ± 1 ppt (final intermolt isoosmotic range / premolt to molt). The best value of molting frequency was in the S4 treatment (29 ± 1 ppt) (close to isoosmotic molt) 10 times. In the maintenance of L. vannamei which is affixed, it is better to pay attention to the needs of isoosmotic media, ie media with a salinity of 25 ± 1-29 ± 1 ppt (final intermolt isoosmotic range / premolt to molt). The best value of molting frequency is in the treatment of S4 (29 ± 1 ppt) (close to isoosmotic molt) 10 times. In the maintenance of L. vannamei which is affixed, it is better to pay attention to the needs of isoosmotic media, ie media with a salinity of 25 ± 1-29 ± 1 ppt (final intermolt isoosmotic range / premolt to molt).

 

Keyword : Salinity, Osmoregulation, Molting, L. vannamei, Ablation.

 

References

Anggoro, S. 1990. The Relationship Between Molting and Osmoregulation in Tiger Shrimp (Penaeus monodon Fab). Postgraduate School, Bogor Institute of Agriculture, Bogor.

Anggoro, S and K. Nakamura. 1996. Osmoregulation of Kuruma Prawn (Penaeus Japonicus). Bull Kagoshima, 2 (3): 14-19.

Beltz, BS 1988. Crustacean Neurohormones. In: Endocrinology of Selected Invertebrate Types, Endocrinology Invertebrates, Vol. 2. Alan R. Liss, New York, pp. 235-258.

Bray, W. A and A. Lawrence. 1992. Reproduction in Penaeus Species in Captivity. In: marine shrimp Culture: Principles and Practices. Fast, AW, Lester, J. (ed.) Elsivier, Amsterdam, pp. 93-170.

Chang 1992. Endocrinology. In: Marine Shrimp Culture: Principles and Practices. Fast, AW, Laster, J. (Ed.) Elsevier, Amsterdam, pp. 53-91.

Chang, ES and JD O'Connor. 1988. Crustacean Molting. In: Endocrinology of Selected Invertebrate Types, Endocrinology Invertebrates, Vol. 2. Alan R. Liss, New York, pp. 259-278.

Che Mat, CR 1987. Study of Macrobrachium rosenbergii's Ecophysiology and Biochemistry and their Relationship with Aquaculture. Collection of Reports on the Use of Physical Sciences Faculty of Science and Gunaan, Kebangsaan University Malaysia. Bangi

Dwiloka, B and B. Srigandono. 2006. Research Methodology. Diponegoro University, Semarang, 219 p.

Ferraris, RP, EDP Estepa, JM Ladja and EGD Jesus. 1986. Osmoregulation in Penaeus monodon: Effects of Molting and External Salinity. p: 637-640. In JL Maclean et. al., eds. Asian Fish. Soc., Manila.

Haefner. Jr. AP 1969. Osmoregulation of Crangon septemspinosa Say (Crustaceans: Caridae). Biol Bull., 137: 438-446.

Hesni, M. A ,, N. Shabanipour, A. Atabati and A. Bitaraf. 2008. Influence of Eyestalk Ablation and Temperature on Molting and Mortality of Narrow-clawed Crayfish (Astacus leptodactylus). J. Fish Aqua Sciences., 8: 219-223.

Kordi, MGH, and AB Tancung. 2007. Water Quality Management in Aquaculture. Rineka Cipta, Jakarta, 208 p.

Mantel, L. H and LL Farmer. 1983. Osmotic and Ionic Regulation, p: 53-161. In Mantel, (ed) The Biology of Crustaceans, Vol.5 Academic Press, New York.

Meade, M. and S. Watts. 2001. Physiological Compensation in Unilateral Eyestalk Ablated Crayfish, Cherax quadricarinatus. J. Exp. Zool, 289: 184-189.

Sainz-Hernandez, JC, IS Racotta, S. Dumas and J. Hernandez-Lopez. 2008. The effects of Unilateral and Bilateral Eyestalk Ablation in Litopenaeus vannamei Male and Female on Several Metabolic and Immunologic Variables. J. Aqua., 383 (1-4): 188-193.

Venkitraman, PR, KV Jayalaksmy, T. Balasubramanian, N. Maheswari, and KKC Nair. 2004. Effect of Eyestalk Ablation on Molting and Growth of Penaeid Prawn: Metapenaeus dobsoni (de Man). Indian J. Exp. Biol, 42: 403-412.

Downloads

Published

2020-05-06

Issue

Vol. 4 No. 2 (2020): Journal of Green Science and Technology, Vol.4 No.2 (2020)

Section

Artikel

Citation Check

License

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share and adapt the work with an acknowledgement of the works authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journals published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Upon receiving the proofs, the Author/Editor agrees to promptly check the proofs carefully, correct any errors, and authorize the publication of the corrected proofs.