Efektivitas Pemberian Ekstrak Almond terhadap Jumlah Morfologi Sperma Mencit Jantan Putih (Mus musculus) Galur Swiss Webster yang Dipapar Asap Rokok
Abstract
ABSTRAK
Latar Belakang : Spermatozoa merupakan sel yang dihasilkan oleh fungsi reproduksi pria. Salah satu kelainan yang terkait yaitu infertilitas dengan penyebab utamanya merokok. Akibat hipoksia jaringan dikarenakan karbon monoksida dari asap rokok sehingga radikal bebas meningkat pada jaringan reproduksi. Antioksidan memiliki peran yang fundamntal dalam eliminasi dari radikal bebas di dalam jaringan, mengurangi apoptosis yang disebabkan oleh stres oksidatif. Kandungan antioksidan pada kacang almond berpengaruh dalam proses perbaikan pembentukan sperma dan morfologi dari sperma.Oleh karena itu perlu diteliti mengenai efektivitas pemberian ekstrak almond terhadap morfologi sperma yang dipapar asap rokok.Tujuan : Mengetahui efektivitas pemberian ekstrak kacang almond terhadap morfologi sperma pada mencit jantan yang dipapar asap rokok.Metode : Penelitian ini merupakan penelitian eksperimental dengan desain -Post test only design. Sampel adalah 24 ekor mencit putih dengan kriteria tertentu, dibagi secara acak menjadi 4 kelompok. Kelompok Kontrol (akuades, tanpa asap rokok), Kelompok Perlakuan 1 (akuades, dengan asap rokok), Kelompok Perlakuan 2 (asap rokok dengan akuades dan ekstrak kacang almond dengan dosis 7 mg/20 g), dan Kelompok Perlakuan 3 (asap rokok, ekstrak akuades dan kacang almond dengan dosis 15 mg/20 g). Pemberian dilakukan secara sonde oral.Hasil : Hasil menunjukan adanya penurunan jumlah morfologi sperma pada KP1 dibandingkan dengan KK (21.190±0.323 dan 37.128±0.1877% dengan p <0.001). peningkatan jumlah morfologi sperma normal pada KP2 dan KP3 (32.1043±0.54 dan 35.3771±0.93 dengan p <0.001). Kesimpulan : Ekstrak kacang almond mempunyai efektivitas dalam meningkatkan jumlah morfologi sperma mencit jantan putih yang dipapar asap rokok.
Kata Kunci: Almond, Antioksidan, Ekstrak Almond, Spermatozoa, Morfologi
ABSTRACT
Background : Spermatozoa are cells produced by the male reproductive system. Infertility, a common disorder of the reproductive system, is known to correlate with cigarette smoke. Carbon monoxide from cigarette smoke may cause tissue hypoxia, leading to an increase of free radicals in the system. Antioxidants have a fundamental role in the elimination of free radicals inside the tissues and reducing oxidative stress-related apoptosis. The antioxidants in almond nuts may have an impact towards sperm production and morphology. Thus, its effectiveness towards sperm morphology in subjects previously exposed to cigarette smoke must be studied. Goals : To determine the efficacy of almond extract administration towards sperm morphology in male rats previously induced with cigarette smoke. Method : This study is an experimental study with a post-test only design. A total of 24 white rats with certain criteria were used as samples and randomly divided into 4 groups; control group (aquadest, no cigarette smoke), treatment group 1 (aquadest, with cigarette smoke), treatment group 2 (cigarette smoke, aquadest, 7 mg/20g of almond extract), and treatment group 3 (cigarette smoke, aquadest and 15 mg/20 g of almond extract). All treatments were administered using oral sonde. Results : We observed a decrease of normal-morphology sperm in KP1 compared with KK (21.190±0.323 and 37.128±0.1877% with a p <0.001). We observed an increase of normal-morphology sperm in KP2 and KP3 (32.1043±0.54 and 35.3771±0.93 with a p <0.001). Conclusion : Almond nut extract has an effectiveness in increasing the morphology of sperm white mice exposed to cigarette smoke.
Keywords: Almond, Almond extract, Antioxidant, Morphology, Spermatozoa.
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